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. 2019 Feb 21;4(4):e124522. doi: 10.1172/jci.insight.124522

Figure 2. Malat1 knockdown attenuates proinflammatory activation of macrophages.

Figure 2

(A) BMDMs were transfected with 20 nM control (con) GapmeR or Malat1 GapmeR. Forty-eight hours after transfection, the cells were treated with or without 100 ng/ml LPS for 6 hours. Total RNAs were isolated and levels of Malat1 determined by real-time PCR. n = 4; mean ± SD. (B and C) Experiments were performed as in A. mRNA (B) and protein (C) levels of the indicated proinflammatory cytokines were determined by real-time PCR or ELISA. n = 3–4; mean ± SD. (D) Experiments were performed as in A. Bacterial killing assay was performed as described in Methods. n = 3; mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001 by 1-way ANOVA with Bonferroni’s post hoc test (A–D). Representative of more than 3 independent experiments.