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. 2019 Feb 21;4(4):e124522. doi: 10.1172/jci.insight.124522

Figure 6. Malat1 regulation of alternative activation of macrophages is dependent on glucose metabolism.

Figure 6

(A) BMDMs were transfected with 20 nM control (con) GapmeR or Malat1 GapmeR. Forty-eight hours after transfection, the cells were pretreated with vehicle or 2 mM 2-DG for 1 hour, followed by treatment with 5 ng/ml IL-4 for 12 hours. Levels of the indicated M2 markers were determined by real-time PCR. n = 4; mean ± SD. (B) BMDMs were transfected with 20 nM con GapmeR or Malat1 GapmeR. Forty-eight hours after transfection, the cells were treated with or without 5 ng/ml IL-4 for 12 hours. Levels of MPC1 and MPC2 were determined by real-time PCR. n = 4; mean ± SD. (C and D) The experiments were performed as in A, except that the cells were pretreated with vehicle, 50 μM UK-5099 (C), or 200 nM rotenone (D) for 1 hour. n = 3–4; mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001 by 1-way ANOVA with Bonferroni’s post hoc test (A–D). Representative of 2 to 4 independent experiments.