Figure 6. Wake-up therapy is a promising strategy to prolong overall survival of breast cancer patients.

(A) Experimental strategy for analysis of residual DTCs at 42 days after surgical resection of primary tumors formed in NOD-SCID mice by Fbxw7 conditional KO MDA-MB-231-mVenus cells and treatment with tamoxifen (TAM) to induce FBXW7 deletion and with paclitaxel (PTX). (B and C) Representative FACS analysis (B) and quantification (C) of remaining DTCs in BM of mice (n = 9 or 10) as in A. Box-and-whisker plots show the median, the lower and upper quartiles, and the minimum and maximum values. *P < 0.05 by 1-way ANOVA. NS, not significant.