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. 2019 Feb 21;4(4):e126444. doi: 10.1172/jci.insight.126444

Figure 9. Inhibitors of K+ channels and CFTR Cl– channels reduce afatinib-induced diarrhea in rats.

Figure 9

(A) Afatinib diarrhea model in Sprague-Dawley rats administered afatinib orally (60 mg/kg, daily). (Left) stool water content (percentage water) as measured daily. (Right) increase in stool water content in individual rats (stool water at indicated data minus stool water on day 0 in same rats). (B) Rats were administered afatinib starting on day 1 and treated starting on day 2 with BPO-27 (10 mg/kg, twice daily, intraperitoneally) or vehicle (mean ± SEM, n = 5 rats per group). (C) Similar protocol as in (B), except for treatment with clotrimazole (100 mg/kg, oral, in 2 divided doses) or vehicle (mean ± SEM, n = 6 rats per group). (D) Similar protocol as in (B), except for treatment with senicapoc (30 mg/kg, oral, twice daily) or vehicle (mean ± SEM, n = 5 rats per group). (E) Similar protocol except for treatment starting on day 1 with clotrimazole and BPO-27, same doses as in (B) and (C), or vehicle (mean ± SEM, n = 5 rats per group). *P < 0.05, **P < 0.01, comparing with day 0 in (A) and with versus without drug treatment in B–E.