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. 2019 Feb 21;4(4):e124710. doi: 10.1172/jci.insight.124710

Figure 3. Bone marrow–derived cells overexpressing Srit2 have enhanced susceptibility to DRA challenge.

Figure 3

Bone marrow chimeras were generated by transplanting WT or Tg Sirt2 bone marrow cells (CD45.2) into lethally irradiated CD45.1 mice. After completion of bone marrow reconstitution (6 weeks), mice were sensitized and challenged. Left lung tissue was isolated for histology, and lung cells from right lobes were isolated via collagenase digestion. (A) The total number of CD45.2 cells was quantified in the lung tissue. n = 4 mice/group; analyzed by 1-way ANOVA. (B) Cellularity of the BAL was determined by flow cytometric staining and graphed as the percentage of donor-derived CD45.2 cells. n = 4 mice/group; analyzed by 1-way ANOVA. (C) Whole lung histological sections were PAS stained. The images are representative of 4 experiments. Scale bar: 2 mm (left); 1 mm (top right); 200 μM (bottom right). (D) Production of CCL17 was assessed in BAL fluid by ELISA. n = 4 mice/group; analyzed by unpaired t test. *P < 0.05, **P < 0.01, ****P < 0.005 when compared with WT control.