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. 2019 Feb 21;4(4):e124710. doi: 10.1172/jci.insight.124710

Figure 4. Adoptive transfer of Sirt2-overexpressing transgenic lung macrophages exacerbates DRA challenge.

Figure 4

Lung macrophages from WT or Tg Sirt2 mice were transferred i.n. into sensitized CD45.1 mice on day 8. Both groups of mice were then challenged with DRA on day 12–14. On day 15, left lung tissue was isolated for histology, and lung cells from right lobes were isolated via collagenase digestion. (A) Relative percentages of different subpopulations of lung immunological cells were assessed by flow cytometry, as described in Supplemental Figure 2. The number of cells was quantified based on the number of recipient CD45.1+ cells. n = 5 mice/group; analyzed by 1-way ANOVA. (B) Lung histological slides were stained with PAS to determine goblet cell hyperplasia. The images are representative of 5 experiments. Scale bar: 2 mm (left); 1 mm (top right); 200 μM (bottom right). (C) Expression of CCL17 was assessed in whole lung tissue by qPCR, and production of CCL17 in BAL fluid was measured by ELISA. n = 5 mice/group; analyzed by 1-way ANOVA. (D) Expression of markers of alternatively activated macrophages, Fizz1 and YM1, was determined in lung tissue after DRA challenge was assessed by qPCR. n = 5 mice/group; analyzed by 1-way ANOVA. ****P < 0.001 when compared with WT control; ###P < 0.001, ####P < 0.0001 when compared within the groups.