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. 2019 Feb 21;4(4):e124710. doi: 10.1172/jci.insight.124710

Figure 5. Sirt2 plays important role on alternative macrophage activation and CCL17 expression.

Figure 5

Primary human alveolar macrophages were isolated via ex vivo lavage from healthy donors. (A) Alveolar macrophages were stimulated with IL-4, IL-13 or IFN-γ for 48 hours and expression of Sirt2 isoforms were detected with either N-terminus or C-terminus specific antibodies. Blot represents 1 set of patient samples, but performed on 3 separate patients. (B) Human AMs macrophages were incubated with rhIL-4 (20 ng/ml) for 24 hours in the presence of AGK2 and expression of CCL17, and (C) alternative activation macrophage markers Arg1 and MRC1 were assessed by qPCR. n = 3 samples/group; analyzed by 1-way ANOVA. *P < 0.05, **P > 0.01, ***P < 0.005, ****P < 0.001 when compared with untreated; ##P < 0.01, ####P < 0.001 when compared across groups.