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. 2019 Apr 11;8:e45973. doi: 10.7554/eLife.45973

Figure 3. Transcriptional activation of endogenous genes by the CRISPR-assisted trans enhancer.

294T, HepG2 and PANC1 cells were transfected with three different transcriptional activation systems to activate expression of 10 endogenous genes. Gene transcription was detected by qPCR and the expression level is shown as the relative RNA expression fold to house-keeping gene GAPDH. Data are shown as mean ± SD, n = 3. The statistical difference was analyzed using the Student’s t test. *, p<0.05; **, p<0.01; NS, no significant statistical difference. Transfection: DVS, dCas9-VP64/sgRNA; DSC, dCas9/csgRNA2-sCMV; DVSC, dCas9-VP64/csgRNA2-sCMV.

Figure 3.

Figure 3—figure supplement 1. Transcriptional activation of endogenous genes by the CRISPR-assisted trans enhancer.

Figure 3—figure supplement 1.

A549, HeLa, SKOV, and HT29 cells were transfected with three different transcriptional activation systems to activate the expression of 10 endogenous genes.
Figure 3—figure supplement 2. Activation of endogenous HNF4α gene in 293 T cell with trans enhancers based on dCas9-VP64 and dCas9-VPR.

Figure 3—figure supplement 2.

The 293 T cell was transfected with various vectors to activate the endogenous HNF4α gene. The HNF4α and GAPDH genes were detected with qPCR.