Figure 2.

This graphically illustrates relative levels of collagen type I (Figure 2a), collagen type III (Figure 2b), fibronectin (Figure 2c) and periostin (Figure 2d) proteins in conditioned medium of adult cardiac fibroblasts following TGF-β1 and/or sulforaphane treatment for 48 hours. The insets show representative immunoblots with the respective primary antisera. Lanes on the insets are: 1) untreated controls, 2) treatment with TGF-β1 alone, 3) treatment with TGF-β1 and 10 μM sulforaphane and 4) treatment with TGF-β1 and 20 μM sulforaphane. Quantitative data are presented relative to untreated control cells. Significance (p ≤ 0.05) was determined by Anova comparison to untreated control cells (*) or to TGF-β1 treatment alone (#). n = 3 independent sets of experiments.