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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Kidney Int. 2019 Feb 28;95(5):1153–1166. doi: 10.1016/j.kint.2018.11.041

Figure 3: iNP-like cells contribute to nephrons in mouse kidney in vivo.

Figure 3:

(A) Schematic of the neonatal injection assay used to determine iNP differentiation capacity. (B) Immunofluorescence of sections through mouse kidneys at 3 and 7 days post-injection with iNP-like cells or control HK2-mCherry cells (stained with HuMt, red). Arrows depict examples of integrated HuMt+ cells or HuMt+ tubules. Scale bars represent 30 μm. (C) Immunofluorescence analysis and detection of fluorescence microsphere auto-fluorescence in neonatal kidneys injected with HK2-mCherry control cells and harvested 7 days post-injection. Arrows depict examples of fluorescent microspheres (green). Scale bars represent 30 μm. Dashed lines in A and C indicate the outer edge of the kidney cortex.