Skip to main content
. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Kidney Int. 2019 Feb 28;95(5):1153–1166. doi: 10.1016/j.kint.2018.11.041

Figure 6: Refinement of reprogramming factors and conditions enables the generation of iNPlike cells from primary human proximal tubule cells (hRPTECs).

Figure 6:

(A) Brightfield images of parental (untransfected) hRPTECs and hRPTEC iNPs that have undergone transfection with pT-SES, pT-TetON and pEF1-HA-m7pB followed by reprogramming. (B) qRT-PCR analysis of NP gene expression in hRPTEC iNP-like cells relative to parental hRPTECs. Data are presented as mean ± SEM. Statistical significance was determined by Student’s t-test (**P≤0.01, n = 3 biological replicates). (C) Immunofluorescence of the mouse kidney organoid assay demonstrating the contribution of hRPTEC iNP-like cells to developing nephrons marked by E-Cadherin (ECad; 692 green). Arrows depict examples of integrated hRPTEC iNP-like cells marked by human nuclear 693 antigen (HuNu; red) and DAPI (nuclei; blue). Scale bar represents 30 μm.