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. Author manuscript; available in PMC: 2019 Sep 28.
Published in final edited form as: Obesity (Silver Spring). 2019 Mar 28;27(5):793–802. doi: 10.1002/oby.22441

Figure 1. Illustration of experimental design.

Figure 1.

Male and female C57BL/6J mice fed 2920x chow diet were subjected to a cross-over design experiment, evaluating metabolic phenotypes exhibited in OxyMax (Columbus Instruments International) and Promethion (Sable Systems International) multiplexed systems. Mice were examined either in OxyMax or Promethion systems at 10 weeks of age, then examined in the opposite system at 11 weeks of age. Mice were then switched to a high fat diet, providing 60% of calories from fat (60% HFD). At 16 weeks of age, mice were again examined in the same system as employed at 10 weeks of age. Body composition was assessed using NMR at 12 weeks of age before initiating 60% HFD, and immediately before and after multiplexed system phenotyping in week 16.