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Detection of acrosome-intact (AI) and acrosome –reacted (AR) sperm via FITC-PNA staining of the acrosomal matrix. a) An AI sperm is seen with the green FITC-PNA staining (arrowed) of the acrosomal matrix. b) An AR sperm is seen without the acrosomal matrix staining. Phase contrast images are seen below sperm with the acrosomal matrix present/absent, followed by the merged images.
