Table 1.

Rates of acrosome-reacted sperm after co-incubation of sperm with PBS (as control) or VGS re-constituted in PBS.

Incubation time (min)	Number (%)
	Status	Control	VGS
0	AR	115 (32.03)	107 (31.02)a,d,f
	No AR	306	316
	Total	421	423
10	AR	91 (25.78)b	405 (83.97)a,b
	No AR	328	88
	Total	419	493
20	AR	104 (30.98)c	429 (92.37)c,d
	No AR	324	49
	Total	428	478
30	AR	123 (28.12)e	403 (95.36)e,f
	No AR	348	20
	Total	471	423
Grand Total	AR	433 (29.23)g	1344 (75.68)g
	No AR	1306	473
	Total	1739	1817

AR: Acrosome reaction. Identical letters refer to significant differences.

^aP<0.001: between before and after 10 min co-incubated with VGS;

^bP<0.001: between co-incubation with PBS as a control and VGS for 10 min;

^cP<0.001: between co-incubation with PBS as a control and VGS for 20 min;

^dP<0.001: between before and after 20 min co-incubated with VGS;

^eP<0.001: between co-incubation with PBS as a control and VGS for 30 min;

^fP<0.001: between before and after 30 min co-incubated with VGS;

^gP<0.001: between the grand control and co-incubated with VGS.