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. Author manuscript; available in PMC: 2020 Jun 20.
Published in final edited form as: Gene. 2018 Oct 23;702:194–204. doi: 10.1016/j.gene.2018.10.049

Fig. 9. The red wines inhibit RNA Pol III-dependent transcription.

Fig. 9.

MCF-7 (A) and KYSE-510 (B) cells were starved in Free/FBS media for 4 hours. Cells were treated with 25mM ethanol and similar amount and three red wines which amounts are equal to ethanol (mM/L) as indicated. RNA was isolated from these cells and RT-qPCR was performed to measure the amounts of pre-tRNALeu (A and B, Left panel) and 5S rRNA (A and B, Right Panel). The fold change was calculated by normalizing to the amount of GAPDH mRNA. The bars represent Mean ± SE of at least three independent determinations.