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. Author manuscript; available in PMC: 2020 Apr 23.
Published in final edited form as: Biomater Sci. 2019 Apr 23;7(5):1888–1897. doi: 10.1039/c8bm01643g

Figure 2. Evaluation of inflammasome activation by PEG-b-(DMAEMA-co-BMA) polymers.

Figure 2.

(A) Secretion of IL-1β by THP-1 and THP1-Def-NLRP3 cells treated with LPS (100 ng/mL) for 3 hours and then indicated polymer solution (10–40 μg/mL) for 4 hours as measured with ELISA (n=6; two-way analysis of variance (ANOVA); p-values correspond to comparison between THP1-Def-NLRP3 and THP-1 at same polymer concentration). (B) IL-1β secretion from THP-1 cells treated with LPS (100 ng/mL) for 3 hours and then polymer solutions (10–40 μg/mL) for 4 hours with or without pre-treatment with Z-VAD-FMK (caspase inhibitor), measured with ELISA (n=6; two-way analysis of variance (ANOVA); p-values correspond to Z-VAD-FMK compared to no inhibitor at same concentration for each polymer). (C) Effect of LPS priming on IL-1β secretion in THP-1 cells treated with or without LPS (100 ng/mL) for 3 hours and then 40 μg/mL of polymer solutions for 4 hours, measured with ELISA (n=6; two-way analysis of variance (ANOVA); p-values correspond to no LPS priming compared to LPS priming for each polymer). (D) Cytotoxicity of polymer solutions (40 μg/mL) in THP-1 cells (4 hour treatment), measured by flow cytometry using Calcein (live) AM/Ethidium Homodimer-1 (dead) cell staining (n=2; one-way analysis of variance (ANOVA); p-values correspond to percentage of cell death caused by each polymer compared to no treatment (NT)). *p<0.05, **p<0.01, ***p<0.005 ****p<0.001.