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. Author manuscript; available in PMC: 2020 Apr 23.
Published in final edited form as: Biomater Sci. 2019 Apr 23;7(5):1888–1897. doi: 10.1039/c8bm01643g

Figure 4. Evaluation of luciferase knockdown and inflammasome activation with siRNA-loaded carriers.

Figure 4.

(A) Secretion of IL-1β by THP-1 cells treated with LPS (100 ng/mL) for 3 hours and then indicated carriers complexed with siRNA (0 – 50nM siRNA) for 4 hours, measured with ELISA (n=6). (B) Secretion of IL-1β by THP-1 cells treated with LPS (100 ng/mL) for 3 hours and then empty polymeric carriers or carriers complexed with siRNA at dose corresponding to 50 nM siRNA for 4 hours, measured with ELISA (n=6). Polymer concentration is matched for both groups ranging from 18 μg/mL (0B) to 38 μg/mL (60B). (C) Percentage of luminescence from MDA-MB-231-Luc cells following 24 hour treatment with indicated carrier complexed to luciferase siRNA, normalized to treatment with the same carrier loaded with scrambled siRNA (n=4; one-way analysis of variance (ANOVA); p-value corresponds to percent luminescence for each polymer compared to percent luminescence of NT, for each concentration independently). *p<0.05, **p<0.01, ***p<0.005 ****p<0.001.