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The CiMRF N-terminus confers myogenicity on Nautilus. Embryos electroporated with pFCiMRFb (A) and pFCiNTNau (B) express SMYD1 in the endoderm, whereas embryos electroporated with pTTFLacZ (C) and pFNau (D) do not. Flag-tagged Nau protein and Flag-tagged CiMRF-Nau chimeric protein is present in endodermal cell nuclei of embryos electroporated with pFNau (E) and pFCiNTNau (F). Size bar is 50μm, and all embryos shown are C. intestinalis.
