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Quantification of Bra expression.
| Bra+ Cells/Embryo | pTLacZ Bra+ Embryos |
pTCiMRFb Bra+ Embryos |
|---|---|---|
| 0 | 0 | 46 |
| 1 | 4 | 25 |
| 2 | 6 | 45 |
| 3 | 21 | 14 |
| 4 | 151 | 15 |
| 5 or 6 | 0 | 0 |
| Total | 182 | 145 |
| Mean | 3.75±0.62 | 1.50±1.31 |
Embryos electroporated with either pTLacZ or pTCiMRFb were cleavage-arrested at the 64-cell stage (approximately 4.25 hours post-fertilization) and assayed for Bra activity by ISH at 11 hours post-fertilization.
