Table 3.

Ectopic CiMRF elicits miR-1/133 expression in the notochord and endoderm.

Exp.#	pTLacZ	pTCiMRFb	pFLacZ	pFCiMRFb
1	4.1 ± 0.9 (31)	6.3 ± 1.4 (28)	0/51	33/63
2	5.6 ± 0.9 (22)	7.6 ± 1.6 (36)	0/39	42/93
3	5.2 ± 1.3 (44)	8.1 ± 2.1 (74)	1/58	34/96

Results of three experiments showing the mean number of miR-1/133 positive cells per cleavage arrested embryo in zygotes electroporated with pTLacZ or pTCiMRFb (number of embryos examined is shown in parentheses). The number of embryos positive for miR-1/133 transcripts in the head endoderm /total number of embryos examined is shown for zygotes electroporated with pFLacZ and pFCiMRFb. In the third experiment with embryos electroporated with pFLacZ, three of the 58 embryos were scored as miR-1/133 positive in the posterior-lateral region of the head.