Table 1.
Primer sets and protocols used for PCR amplification of blood-borne agents in dogs from Crete, Greece
| Target organism | Genetic marker | Primer sequences (5′ → 3′) | Product size (bp) | Reference |
|---|---|---|---|---|
| Leishmania infantum | kDNA | RV1: CTTTTCTGGTCCCGCGGGTAGG RV2: CCACCTGGCCTATTTTACACCA |
145 | le Fichoux et al. 1999 |
| ssu-rRNA | Lei70L: CGCAACCTCGGTTCGGTGTG Lei70R: CGCGGTGCTGGACACAGGGTA |
345 | Spanakos et al. 2002 | |
| cpb | Leicpb_for: CGTGACGCCGGTGAAGAAT Leicpb_rev: CGTGCACTCGGCCGTCTT |
702 | Hide and Bañuls 2006 | |
| Babesia/Hepatozoon | 18S rRNA | BTH-1F: CCTGAGAAACGGCTACCACATCT BTH-1R: TTGCGACCATACTCCCCCCA Nested GF2: GTCTTGTAATTGGAATGATGG GR2: CCAAAGACTTTGATTTCTCTC |
720/750 590/610 |
Zintl et al. 2011 |
| Hepatozoon spp. | 18S rRNA | H14Hepa18SFw: GAAATAACAATACAAGGCAGTAAAATGCT H14Hepa18SRv: GTGCTGAAGGAGTCGTTTATAAAGA |
620 | Hodžić et al. 2015 |
| Anaplasmataceae | 16S rRNA | EHR16SD: GGTACCYACAGAAGAAGTCC EHR16SR: TAGCACTCATCGTTTACAGC |
345 | Brown et al. 2001 |
| Hemotropic mycoplasmas | 16S rRNA | HBT-F: TACGGCCCATATTCCTACG HBT-R: TGCTCCACCACTTGTTCA |
600 | Criado-Fornelio et al. 2003 |
| Filarioid nematodes | COI | H14FilaCOIFw: GCCTATTTTGATTGGTGGTTTTGG H14FilaCOIRv: AGCAATAATCATAGTAGCAGCACTAA |
724 | Hodžić et al. 2015 |