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. 2019 Apr 17;10:778. doi: 10.3389/fmicb.2019.00778

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Copyright © 2019 Iradi-Serrano, Tola-García, Cortese and Ugalde.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypes and C/area (millions/cm²) ratios of all the FluG mutants. (A) Wild type (WT) along with fluG null (ΔfluG), N-terminal (aa1–aa406), C-terminal (aa427–aa865) regions and N- and C-terminal separated regions (N+C separated) mutants in MMA and CMA. (B) FluG point-mutations in both regions. (C) Heterologous substitutions, showing LSEI_0440+C-term (FluG), N-term (FluG)+PA5508, LSEI_0440+PA5508 and PA5508 only. Note that different scales were used in each panel to better represent the differences in C/area (millions/cm²) values. The C-values that are statistically different (P-value < 0.05) to the WT (^∗) and the ΔfluG (#) are marked. Scale bar = 1 cm.