Fig. 7.

DTM defines the SlCLV3 and SlWUS expression domains. a, b expression of DTM and SlREV in wild type (Moneymaker) shoot apices as revealed by RNA in situ hybridization. c, d expression patterns of the meristematic SlCLV3, SlWUS and SlSTM genes in the shoot apices of dtm-1 (c), dtm-cr5 (d), and their corresponding wild types (LA2397 and Moneymaker) at 6 days after germination (DAG) determined by quantitative reverse transcription (qRT)-PCR. SleIF4α6 was used as the reference gene to normalize gene expression. Data represent means ± sem, n = 6 biologically independent samples. A two-tailed t-test was applied to compare the differences in means between dtm-1 and wild type. e expression patterns of the SlCLV3, SlWUS, and SlSTM genes in the shoot apices of dtm-1, dtm-cr5 and wild type (Moneymaker) at 6 DAG revealed by RNA in situ hybridization. Scale bars, 100 μm. f expression levels of DTM and the meristematic SlCLV3, SlWUS, and SlSTM genes in slrev-cr2 shoot apices and wild type (Moneymaker) at 6 DAG determined by qRT-PCR. Data represent means ± sem, n = 5 (wild type) or 7 (slrev-cr2) biologically independent samples. Fold changes (FC, mutant vs wild type (WT)) of gene expression levels are given under the graph. A Welch’s t-test was applied to compare the differences in means between slrev-cr2 and wild type. g–r expression of DTM (g–j), SlCLV3 (k–n), and SlWUS (o–r) in slrev-cr2 (i, j, m, n, q and r) and wild type (Moneymaker, h, l and p) shoot apices revealed by in situ hybridization. Hybridization using sense probes served as negative controls (g, k and o). Scale bar, 100 μm. The experiments were repeated at least twice using different batches of plants with similar results. Expression data collected from the dtm-1 and dtm-cr5 seedlings at 3 and 9 DAG are provided in Supplementary Fig. 10c-d. SlSTM expression in slrev-cr2 and wild type apices is available in Supplementary Fig. 10e