Figure 4.

CKD promotes the recruitment of microglia/macrophages within the ischemic penumbra, impairs their M₂- and promotes their M₁-polarization. (A) Immunostaining analysis of Iba-1 expression (a marker of activated microglia) in the striatum (left) and the cortex (right) of SHAM vs CKD mice. Bars: 100 µm. Quantifications represent the number of Iba-1 positive cells per fields of 10000 µm². Results are expressed as mean ± SEM and represent data from at least 8 animals per group. ***p < 0.001 CKD versus SHAM mice (non parametric Mann-Whitney U test). (B) Analysis by real-time PCR of the M₂ markers CD206, ARG1 and Fizz1. (C) Analysis by real-time PCR of the M₁ markers CD32, CD86 and IL-1β. Results are expressed as mean ± SEM and represent data from at least 8 animals per group. *p < 0.05, **p < 0.01, ***p < 0.001 ipsilateral versus contralateral hemisphere (non parametric Wilcoxon matched pairs test). ^$p < 0.05, ^$$p < 0.01, ^$$$p < 0.001 CKD versus SHAM mice (non parametric Mann-Whitney U test). Contro: contralateral hemisphere; Ipsi: ipsilateral hemisphere.