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. 2019 Apr 23;9:6428. doi: 10.1038/s41598-019-41444-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of proteins that regulate apoptosis and cell signaling in Hep3B cells treated with Fraxini. (A) Cell cycle and apoptotic regulating protein in Fraxini treated Hep3B cells by RPPA. (B) Cell signaling proteins, including c-Myc were altered dramatically by Fraxini treatment. (C) Western blot analysis confirmed the protein expression changes observed in RPPA analysis. (D) Immunohistochemical staining of c-Myc protein in Hep3B tumor tissues treated with Fraxini.