Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 23;9:6454. doi: 10.1038/s41598-019-42943-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

irx2a restricts the PST and promotes the DL during pronephros segmentation. (A) Lateral view of WISH analysis with the PST marker trpm7 at the 28 ss. Black bars highlight the trpm7 expression domain in the pronephros. Scale bar is 50 μm. (B) Quantification of trpm7 length (μm). Each dot represents one nephron and data is presented +/− SEM. Statistical significance was determined with ANOVA. (C) WISH in 28 ss embryos with the DL marker slc12a3 shown in a lateral view. Black bars denote slc12a3 expression. (D) Quantification of slc12a3 domain length (μm), where each dot represents one nephron. Data is presented +/− SEM and statistical significance was determined by ANOVA. WISH – whole mount in situ hybridization, ss – somite stage, PST – proximal straight tubule, DL – distal late tubule.