Table 1.
Richness and diversity parameters of gut microbiome from C. maculatus as affected by DDVP and EO.
Generations | F1 | F5 | ||||
---|---|---|---|---|---|---|
Parameters | Control | DDVP | EO | Control | DDVP | EO |
Coverage (%) | 77.08 ± 2.9b | 71.96 ± 1.56bc | 87.38 ± 2.19a | 68.4 ± 1.32c | 72.08 ± 2.24bc | 89.42 ± 1.54a |
OTUs1 | 74 ± 4a | 77 ± 5a | 54 ± 6b | 76 ± 4a | 84 ± 5a | 22 ± 3c |
Chao1 | 7.39 ± 1ab | 7.47 ± 0.04a | 5.93 ± 0.15c | 6.87 ± 0.18b | 7.18 ± 0.1ab | 4.47 ± 0.3d |
Shannon2 | 4.78 ± 0.13a | 4.98 ± 0.1a | 3.58 ± 0.3b | 4.61 ± 0.14a | 4.56 ± 0.15a | 2.47 ± 0.11c |
Simpson2 | 0.92 ± 0.02a | 0.94 ± 0.01a | 0.67 ± 0.03c | 0.82 ± 0.03b | 0.76 ± 0.04d | 0.57 ± 0.03d |
IL (kb)3 | 1.3–1.5 | 1.3–1.5 | 1.3–1.5 | 1.3–1.5 | 1.3–1.5 | 1.3–1.5 |
Cutoffs | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Different letters (a, b, c, d) within rows are statistically different after parametric New Duncan’s Multiple Range Test at P = 0.05. 1The operational taxonomic units (OTUs) were defined with 3% dissimilarity level; 2The richness estimator (Chao1) and diversity indices (Shannon and Simpson) were calculated according to Good’s method72 and the mothur program, respectively; 3Isoform length (kb). Each datum represents the Mean ± SE of 3 replicates.