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. 2019 Apr 23;9:6435. doi: 10.1038/s41598-019-42843-1

Table 1.

Richness and diversity parameters of gut microbiome from C. maculatus as affected by DDVP and EO.

Generations F1 F5
Parameters Control DDVP EO Control DDVP EO
Coverage (%) 77.08 ± 2.9b 71.96 ± 1.56bc 87.38 ± 2.19a 68.4 ± 1.32c 72.08 ± 2.24bc 89.42 ± 1.54a
OTUs1 74 ± 4a 77 ± 5a 54 ± 6b 76 ± 4a 84 ± 5a 22 ± 3c
Chao1 7.39 ± 1ab 7.47 ± 0.04a 5.93 ± 0.15c 6.87 ± 0.18b 7.18 ± 0.1ab 4.47 ± 0.3d
Shannon2 4.78 ± 0.13a 4.98 ± 0.1a 3.58 ± 0.3b 4.61 ± 0.14a 4.56 ± 0.15a 2.47 ± 0.11c
Simpson2 0.92 ± 0.02a 0.94 ± 0.01a 0.67 ± 0.03c 0.82 ± 0.03b 0.76 ± 0.04d 0.57 ± 0.03d
IL (kb)3 1.3–1.5 1.3–1.5 1.3–1.5 1.3–1.5 1.3–1.5 1.3–1.5
Cutoffs 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001

Different letters (a, b, c, d) within rows are statistically different after parametric New Duncan’s Multiple Range Test at P = 0.05. 1The operational taxonomic units (OTUs) were defined with 3% dissimilarity level; 2The richness estimator (Chao1) and diversity indices (Shannon and Simpson) were calculated according to Good’s method72 and the mothur program, respectively; 3Isoform length (kb). Each datum represents the Mean ± SE of 3 replicates.