Table 2.
ID50 values of C. elegans strains after 48 and 72 hours from exposure to UV and gamma irradiation.
| Time post exposure | Treatment | Strain† | ID50‡ | R | Slope ± SE | X 2 | df |
|---|---|---|---|---|---|---|---|
| 48 hrs | UV (J cm−2) | N2 | 51.63 (47.56–57.40) | 0.99 | 3.71 ± 0.42 | 0.18 | 5 |
| dld-1(wr4) | 69.85 (60.64–86.49)*** | 0.99 | 2.90 ± 0.37 | 1.72 | 6 | ||
| SP483 | 36.60 (32.62–41.52)** | 0.98 | 2.12 ± 0.23 | 4.15 | 6 | ||
| SP488 | 10.50 (6.51–13.92)**** | 0.96 | 1.35 ± 0.22 | 2.92 | 5 | ||
| CE1255 | 35.11 (31.08–39.21)** | 0.97 | 2.38 ± 0.35 | 2.95 | 5 | ||
| γ (Gy) | N2 | 276.00 (221.15–355.12) | 0.96 | 1.20 ± 0.14 | 3.61 | 5 | |
| dld-1(wr4) | 320.84 (243.26–455.75) | 0.99 | 0.96 ± 0.14 | 0.05 | 5 | ||
| DW102 | 297.17 (218.61–438.03) | 0.97 | 0.85 ± 0.14 | 2.72 | 5 | ||
| DW103 | 246.68 (128.77–570.69) | 0.95 | 1.60 ± 0.15 | 12.78 | 5 | ||
| CE1255 | 489.37 (331.01–1567.23) | 0.97 | 1.67 ± 0.17 | 11.48 | 5 | ||
| 72 hrs | UV (J cm−2) | N2 | 61.20 (54.39–72.22) | 0.99 | 2.98 ± 0.35 | 2.20 | 6 |
| dld-1(wr4) | 66.15 (57.40–83.39) | 0.96 | 2.77 ± 0.41 | 5.82 | 5 | ||
| SP483 | 48.06§ | 0.87 | 1.86 ± 0.23 | 23.54 | |||
| SP488 | 10.83 (5.49–15.21)**** | 0.93 | 1.03 ± 0.20 | 3.68 | 6 | ||
| CE1255 | 64.52 (54.96–81.27) | 0.96 | 2.21 ± 0.28 | 7.67 | 5 | ||
| γ (Gy) | N2 | 223.28 (171.94–294.31) | 0.99 | 1.02 ± 0.14 | 0.42 | 5 | |
| dld-1(wr4) | 290.63 (229.88–382.56) | 0.99 | 1.13 ± 0.14 | 1.98 | 5 | ||
| DW102 | 205.43 (158.38–267.37) | 0.99 | 1.04 ± 0.14 | 1.52 | 5 | ||
| DW103 | 421.54 (355.28–516.91)*** | 0.96 | 1.80 ± 0.17 | 6.17 | 5 | ||
| CE1255 | 444.46 (369.12–558.06)*** | 0.99 | 1.67 ± 0.17 | 3.09 | 5 |
†N2 (wild type), dld-1(wr4) (phosphine-resistant), SP483 & SP488 (UV-sensitive), DW102 & DW103 (ionizing radiation-sensitive) and CE1255 (resistant to radiation-induced apoptosis).
‡*p < 0.05, **p < 0.005, ***p < 0.001 and ****p < 0.0001.
§Confidence limits could not be computed because G-test is >0.4.
Values were computed from probit analysis for each strain and treatment. The growth-inhibitory effect of each treatment is compared to the unexposed animals as growth reduction percentages. A comparison with one way ANOVA followed by Dunnett’s multiple test was performed to identify significant differences in ID50 values due to exposure of radiation treatments between the wild type and the other strains.