Fig. 2.

SIRT3 is required for behavioral and synaptic adaptations to IF. a Immunoblots show relative levels of SIRT3 protein in hippocampus of mice that had were either fed AL or maintained on alternate day food deprivation (IF) for 1 week or 1 month. The upper blot is samples from mice killed on the second day after diet initiation (at the end of the first 24 h FD period for mice in the IF group). *P < 0.05, ***P < 0.001 compared to the AL NFD and 24 h FD groups (11 mice/group). b, c Time spent (left) and distance traveled (right) in the open arms of the elevated maze (b) and in the center zone of the open field (c) for mice that had either been fed AL or IF for 1 month (12 mice/group). All tests were performed in mice that had been deprived of food for 24 h (FD). *P < 0.05. d Whole-cell recordings of mIPSCs in CA1 neurons in hippocampal slices from mice in the four indicated groups. e mIPSC amplitudes (left) and frequencies (right) of mIPSCs (15 neurons in slices from 5 mice/group). *P < 0.05. f–h fEPSP recordings at CA1 synapses in hippocampal slices from Sirt3^+/+ and Sirt3^–/– mice that had been maintained for 4 months on either AL or IF diets. f shows basal synaptic transmission with the amplitude of the fiber volley plotted against fEPSP slopes (input/output curves). g shows the results of LTP analysis. Panel h shows quantification of LTP magnitude. *P < 0.01 compared to the values for each of other three groups (10 slices from 5 mice/group). i Results of Y-maze testing. j, k Results of water maze testing. Group difference, F (3, 27) = 3.762, P = 0.022. k shows data for escape latencies on testing day 1 trial 4, and testing day 2 trial 1 (left), and latency ratios (escape latency on day 2 trial 1 divided by the escape latency on day 1 trial 4) (right). 8 mice per group, *P < 0.05, **P < 0.01. All error bars are the SEM. All statistical comparisons used ANOVA and Newman–Keuls post hoc tests. Source data are provided in supplemental Source Data file