Figure 10.

The lack of an effect of GnRHR activation on Spp1 expression in vitro (A,B) and in vivo (C). Experiments were performed with 7-week-old females. (A) Pituitary cells were cultured overnight in medium 199 containing horse serum, washed in the morning, and cultured in medium 199 supplemented with BSA with or without 10 nM GnRH for 0–60 h prior to the end of experiments, and samples were analyzed for Dmp1 (top) and Spp1 (bottom) mRNA expression. (B) 96 h old cell cultures were treated as described in (A), up to 48 h, and samples were analyzed for Dmp1 (top) and Spp1 (bottom) mRNA expression. (C) Animals were injected with buserelin acetate or solvent as described above. Gray bars and circles, GnRH/buserelin acetate-treated cells/animals; white bars and circles, solvent-treated cells/animals.