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. 2019 Apr 17;10:248. doi: 10.3389/fendo.2019.00248

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Copyright © 2019 Bjelobaba, Janjic, Prévide, Abebe, Kucka and Stojilkovic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Triple immunofluorescence labeling for OPN (green), FSHβ (red), and LHβ (blue) in adult male rat pituitary tissue. Low magnification images (upper row) show that all OPN-positive cells were also LHβ-positive; most of the OPN-positive cells are both FSHβ and LHβ-positive. However, OPN was not visible in all gonadotrophs. Scale bar: 100 μm. High -magnification of triple immunofluorescence labeling for OPN, FSHβ and LHβ (bottom row) shows a cluster of gonadotroph cells, most of which show immunoreactivity for all three markers. Scale bar: 10 μm.