Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The lack of an ability of GnRH to stimulate Spp1 expression in cultured anterior pituitary cells. Experiments were performed with cells from 4-week-old females and males. Cells were cultured overnight in medium 199 containing horse serum, which was replaced with BSA-containing medium 199 supplemented with 10 nM GnRH in the morning, cultured for 0 to 8 h, and mRNA was extracted for measurement of Spp1 (A), Dmp1 (B), and Gnrhr (C) expression.
