Fig. 6.

Assay to measure asexual-to-sexual-stage conversion. a Schematic of the experimental approach. HCT-8 cells were infected for 48 h, after which experimental compounds were added and the cultures were incubated for an additional 24 h before immunofluorescence staining with V. villosa lectin and anti-DMC1 antibody, and high-content microscopy. b Representative dose-response curves for the asexual growth assay (i.e., first 48 h) and the sexual development assay (i.e., inhibition of DMC1 expression from 48 to 72 h). Examples are shown for the piperazine compound D1 (MMV665917) and for the 2,4-diaminoquinazoline B-1 (MMV006169). c Graph showing the percent inhibition of DMC1 expression for control compounds and the test set of compounds. For b and c the mean and SD combined from at least two biological replicates with four technical replicates per experiment are shown. Source data are provided as a Source Data file