Fig. 7.

Dendrogram showing the results of a clustering analysis based on life cycle stage assay data for a diverse set of 39 Cryptosporidium growth inhibitors. Results from the life cycle stage assays were used to calculate a distance matrix using Euclidean distances from the mean control results, and the dendrogram was generated using the Ward error sum of squares hierarchical method. For the major nodes, bootstrap proportions ≥ 60% are shown (1000 bootstrap iterations). Singleton compounds, i.e., those with no known similarity to other compounds in the collection, are shown in black, and others are colored according to chemotype and/or putative mechanism of action. Compound structures and phenotypic assay results for each are shown in Supplementary Fig. 1 and Supplementary Table 2, respectively. NSG mouse efficacy studies performed on 31/39 compounds are summarized in Supplementary Table 3, and compounds with efficacy in the NSG mouse model at the dose tested are indicated with an arrow. Source data are in Supplementary Table 2