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. 2019 Apr 23;9:6414. doi: 10.1038/s41598-019-42765-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Iron deficiency leads to a higher apoptosis rate of Kit^Pos HPCs compared to Kit^Neg ones. (a) The frequency (%) of apoptotic (AnnexinV⁺ 7AAD⁺) cells is presented as a function of treatment in both Kit^Pos and Kit^Neg HPCs after 24 hours (left panel) and 48 hours (right panel) treatment. *p < 0.05 for control versus DFO or # for DFO versus Fe groups by one-way ANOVA and Tukey multiple comparisons test. (b) The delta apoptosis (% DFO - % control) was compared between Kit⁺ CD41⁺ and Kit⁻ CD41⁺ HPs. *p < 0.05 and **p < 0.01 for Kit⁺ CD41⁺ versus Kit⁻ CD41⁺ by paired two-tailed t-test.