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. 2019 Apr 23;9:6394. doi: 10.1038/s41598-019-42767-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pin1 increases the stability of YAP/TAZ proteins. (A–D) YAP and TAZ are degraded more in Pin1 knockout cells. Equal amount of FLAG-tagged YAP (A, B) or FLAG-tagged TAZ (C,D) was transfected into sgControl or sgPin1 stable HEK293 cell lines separately. 24 hrs after transfection, cells were treated with 100 μg/ml cycloheximide and harvested at different time as indicated in figure. Immublot was carried out using the respective antibodies as shown.