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. 2019 Apr 23;10:1830. doi: 10.1038/s41467-019-09854-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Phenotypic selection of pERK2-specific DARPin binding. a Representative spot titer images and b survival curves for serially diluted E. coli MC4100 cells co-expressing TatABC along with the antigen-Bla reporter fusion (MBP-Bla, ERK2-Bla, or JNK2-Bla) and a Tat-targeted DARPin (spTorA-pE59 or spTorA-OFF7) as indicated. Resistance of cells was evaluated in the presence (+) or absence (−) of MEK1^R4F kinase. Overnight cultures were serially diluted in liquid LB and plated on LB agar supplemented with Carb. Maximal cell dilution that allowed growth is plotted versus Carb concentration. Arrow in b indicates Carb concentration that is depicted in image panel a above the graphs and corresponds to 100 μg/ml Carb. Source data for this figure is available in the Source Data File