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. 2019 Apr 23;10:1887. doi: 10.1038/s41467-019-09763-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Evaluation of cellular fibrin hydrogel formation. a Live-cell confocal fluorescence micrograph (2D) showing Alexa Fluor® 594 labelled fibrin (red) nucleation after 20 min from the surface of hMSCs stained with Calcein AM (green) and Hoechst 33342 (blue). Insert shows single cell at higher magnification. Scale bars represent 100 and 10 μm (insert). b Photographs of the free-standing fibrin hydrogels 24 h after gelation formed via [sc_thrombin][ox890] hMSCs labelling. c Scanning electron micrograph of [sc_thrombin][ox890] modified hMSCs aggregates (3D) after 24 h showing a surface catalysed fibrin matrix. Insert shows cells at higher magnification. Scale bars represent 50 and 5 μm (insert)