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. 2019 Apr 23;9:6424. doi: 10.1038/s41598-019-42377-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Mixture S on testicular histology of PND21 (a) and on testicular histology (b) and sperm content (c) of PND90 offspring exposed to 0x (DMSO), 10x, 100x and 500x SELMA mothers’ levels. Bars represent the estimated marginal means ± SE of the percent of seminiferous tubule types detected in each group. Number of animals used PND21: n = 4–10; PND90: n = 7–13. *p < 0.05; ¤p < 0.01; ^#p < 0.001. Photomicrographs of tubules from DMSO-treated and Mixture S-treated animals at PND21 (a1–a5) and PND90 (b1–b4) showing normal tubules (a1, b1) and representative aberrations detected in experimental animals. a2, b2: no lumen; a3, b3: germ cells in the lumen; a4, b4: detached layers; a5: abnormal layers. Scale bar 20 μm.