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. 2019 Apr 23;9:6424. doi: 10.1038/s41598-019-42377-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Mixture S on ovarian histology of PND90 offspring exposed to 0x (DMSO), 10x, 100x and 500x SELMA mothers’ levels. Bars represent the estimated marginal means ± SE of the number of follicles per mm² of ovary. Significantly reduced number of preantral follicles (primary and secondary) and increased atresia were detected in all treated groups vs. DMSO. *p < 0.05; ^¤p < 0.01; ^#p < 0.001. Number of animals used: 7–12. The phase of the estrous cycle was included as covariate in the statistics. Representative H&E stained sections of the whole ovary are provided per group ((a) DMSO, (b) 10x, (c) 100x, (d) 500x; scale bar 200 μm). Respective framed areas are shown at higher magnification below each group. Scale bar 50 μm. Yellow arrowheads denote typical atretic follicles and asterisks indicate follicles with pyknotic cells.