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. 2019 Apr 23;9:6424. doi: 10.1038/s41598-019-42377-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Mixture S on circulating Testosterone (T), Estradiol (E2) and Luteinizing hormone (LH) levels of PND90 male (a–c) and female (e,f) offspring exposed to 0x (DMSO), 10x, 100x and 500x SELMA mothers’ levels. In (d) the percentage of males per group exhibiting a T/E2*100 value lower than the threshold of 10 (dashed horizontal line) is indicated. Bars represent the estimated marginal means ± SE. Number of males used for T, E2 and LH: 8–16, 7–13 and 3–11, respectively. Number of females used for E2 and LH: 5–13 and 3–6, respectively. Samples of siblings were pooled for LH. In females, the phase of the estrous cycle was included as covariate in statistics. ^¤p < 0.01.