Fig. 1.

Impact of Cox-1/2 deletion on MI/R injury. Infarct size (IS) (a) and area at risk (AAR) (b) were quantified 24 h after I/R injury, as detailed in the Methods. Representative photographs showing TTC stained transverse sections of Evans blue perfused hearts were shown for each study group (c). In a separate study, mice were orally treated with 100 mg/kg celecoxib or vehicle before and 20 h after surgery. Representative TTC staining of the heart sections was shown for each group (c), and their infarct size (d) and AAR (e) were quantified. Biosynthesis of PGE₂ (f) was measured by HPLC-MS-MS as detailed in the Methods. One-way ANOVA with Tukey’s multiple comparison test (a, b, n = 15, 14, 9; d, e, n = 12, 14, 16. Individual n numbers in each panel are shown in same order as their corresponding groups from left to right, and this arrangement is applied to other relevant figures in the text.). Unpaired Student’s t test (f, n = 6). Statistical significance is demarked as *P < 0.05, **P < 0.01, and ***P < 0.001, throughout the text. Error bar indicates SEM