Fig. 4.

mPges-1 deletion and PGE receptor antagonism in arteriolar dilation in I/R. Arterioles of 15–25 μm in diameter were randomly selected in the extremity of hindlimb. Vascular response (percentage change of diameter over baseline) to I/R was assessed for mice deficient in mPges-1 (a). The vessel diameter was not different between control (n = 9) and mPges-1-1 KO (n = 7) mice at baseline (21.34 ± 0.72 vs 20.38 ± 1.40 μm, p = 0.54, unpaired Student’s t test). b The vascular response was also assessed for WT mice i.v. treated with vehicle (n = 6), or an antagonist EP1 (ONO-8130, Ki = 1.9 nM, n = 5), EP2 (PF-04418948, IC50 = 16 nM, n = 5), EP3 (L-798106, Ki = 0.3 nM, n = 7) or EP4 (GW627368X, Ki = 100 nM, n = 7), with each drug at 5 mg/kg. There was no difference in baseline vessel diameter between groups. Two-way ANOVA with Bonferroni’s test was used. * demarks statistical comparison between control and mPges-1 KO, or between GW 627368X and vehicle. # shows comparison to baseline. Error bar indicates SEM