Table 4.

Response and experimental design of enzyme-assisted extraction method to extract TRPs.

Test group	Liquid–solid ratio (X1) (mL/g)	Enzymolysis time (X2) (min)	Enzyme-complex dosage (X3) (%)	pH (X4)	TRPs yields (%)
					Actual value	Predicted value
1	25	60	1.5	6	9.16	9.25
2	35	60	1.5	6	10.81	11.15
3	25	120	1.5	6	11.36	11.04
4	35	120	1.5	6	8.07	8.02
5	30	90	1	5	8.78	8.98
6	30	90	2	5	11.92	11.59
7	30	90	1	7	10.58	10.94
8	30	90	2	7	10.57	10.41
9	25	90	1.5	5	9.09	9.88
10	35	90	1.5	5	10.02	10.31
11	25	90	1.5	7	10.89	11.26
12	35	90	1.5	7	9.82	9.71
13	30	60	1	6	9.18	9.46
14	30	120	1	6	9.57	9.61
15	30	60	2	6	10.69	11.32
16	30	120	2	6	9.44	9.83
17	25	90	1	6	11.31	10.76
18	35	90	1	6	9.33	9.01
19	25	90	2	6	10.99	10.61
20	35	90	2	6	11.39	11.24
21	30	60	1.5	5	11.35	10.57
22	30	120	1.5	5	9.08	8.92
23	30	60	1.5	7	10.53	9.98
24	30	120	1.5	7	10.20	10.29
25	30	90	1.5	6	13.03	13.34
26	30	90	1.5	6	13.07	13.34
27	30	90	1.5	6	13.59	13.34
28	30	90	1.5	6	13.45	13.34
29	30	90	1.5	6	13.56	13.34

The predicted values were not significantly different from the corresponding actual values (P > 0.05).