Fig. 2.

Inactivation of CPu, SSC and thalamus (Thal) suppresses SWDs and activation of CPu induces SWDs in Stxbp1^+/− mice. a Muscimol injections in Stxbp1^+/− mice. (Left) Injections into SSC, CPu, Thal but not in CA1 and mPFC suppressed SWDs in SSC ECoG recordings (3 h recording after injection). SSC injection (N = 5), CPu injection (N = 5), Thal injection (N = 5), CA1 injection (N = 5), mPFC injection (N = 5). Mann–Whitney U test, vehicle vs. muscimol, SSC: **P = 0.0079; CPu: *P = 0.0119; Thal: **P = 0.0079; CA1, P = 0.5476, mPFC P = 0.9166. Numbers of mice are shown in parentheses. (Right) Representative recordings in the mice with CPu injection. SWDs were suppressed in SSC, mPFC and CPu recordings. b Single brief electrical stimulation of the CPu evoked SWDs in Stxbp1^+/− mice. (Left) Representative recordings. (Right) Regional occurrence of spontaneous and evoked SWDs (6 experiments from 5 mice, 1 h recording). Mann–Whitney U test, spontaneous SWDs vs. evoked SWDs, SSC: **P = 0.0022; mPFC: *P = 0.0152; CPu: **P = 0.0022; Thal: P = 0.0571. Mean ± SEM. *P < 0.05, **P < 0.01