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. 2019 Apr 23;10:1917. doi: 10.1038/s41467-019-09954-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Stxbp1 deletions in cortico-striatal but not cortico-thalamic projection neurons causes SWDs. a (Left) Trpc4-Cre dependent tdTomato (red) expression in neocortical layer 5 and the striatum (coronal section). (Right) Ntsr1-Cre dependent tdTomato expression (red) in neocortical layer 6 and the thalamus (coronal section). Scale bars: 500 μm. b Stxbp1^fl/fl/Trpc, but not Stxbp1^fl/fl/Ntsr, mice showed SWDs. (Left) Representative recordings. (Right) SSC SWDs numbers (3 h recording). Mann–Whitney U test, Stxbp1^fl/fl/Trpc (N = 5) vs. control mice (3 Stxbp1^+/+/Trpc, 1 Stxbp1^fl/fl, N = 4), *P = 0.0195; Stxbp1^fl/fl/Trpc (N = 5) vs. Stxbp1^fl/+/Trpc (N = 3), *P = 0.0357; Kruskal–Wallis test, *P = 0.0156, Dunn’s multiple comparison test, Stxbp1^fl/fl/Trpc (N = 5) vs. Stxbp1^fl/+/Trpc (N = 3), *P < 0.05. However, neither Stxbp1^fl/fl/Ntsr (N = 5) nor Stxbp1^fl/+/Ntsr (N = 3) mice increased SWDs, comparable to control mice (1 Stxbp1^+/+, 1 Stxbp1^+/+/Ntsr, 1 Stxbp1^fl/+, N = 3). Kruskal–Wallis test, P = 0.3962. c Scn2a^fl/fl/Trpc, but not Scn2a^fl/fl/Ntsr, mice showed SWDs (3 h recording). Scn2a^fl/fl/Trpc (N = 5) vs. Scn2a^+/+/Trpc (N = 4), Mann–Whitney test, *P = 0.0317. d Injected NeuRet vector (Lenti-FLP) in the striatum is retrogradely transported to the SSC, where FLP activates the Cre recombinase of the injected AAV5-EF1a-fDIO-Cre in the SSC. The Cre recombinase deletes floxed-Stxbp1 and activates tdTomato genes (red). Scale bar: 500 μm. e SWDs appeared in mice with NeuRet-dependent Stxbp1 deletion in cortico-striatal projection neurons (3 h recording). Unpaired t test with Welch’s correction, Stxbp1^fl/fl (N = 6) vs. Stxbp1^fl/+ (N = 3), t₅ = 3.027, *P = 0.0292; Stxbp1^fl/fl (N = 6) vs. Stxbp1^+/+ (N = 3), t₅ = 3.027, *P = 0.0292. Numbers of mice are shown in parentheses. *P < 0.05