Fig. 5.

Competitive inhibition of pacCer photolabeling of VDACs by C₁₆-ceramide. a VDAC1, VDAC1^E73Q, VDAC2, and VDAC1^E84Q proteoliposomes were photolabeled with the indicated amount of pacCer or pacChol added from ethanolic stocks. Next, samples were click-reacted with AF647-N₃, subjected to SDS-PAGE, and analyzed by IGF and CB staining. b VDAC1, VDAC1^E73Q, VDAC2, and VDAC1^E84Q proteoliposomes were photolabeled with 0.2 nmol pacCer or pacChol in the presence of the indicated amounts of natural C₁₆-ceramide (Cer) added from ethanolic stocks and then processed as in a. Relative labeling efficiencies were quantified and expressed as % of control (0.2 nmol pacCer or pacChol in the absence of Cer). Data are means ± s.d.; n = 3; *p < 0.05, **p < 0.01, and ***p < 0.001 by two-tailed paired t-test. Source data