Fig. 7.

Ceramide-induced apoptosis critically relies on Glu84 in VDAC2. a WT and ΔVDAC1/2 HCT116 cells stably transduced with HA-tagged VDAC1, VDAC1^E73Q, VDAC2, or VDAC2^E84Q were transfected with empty vector (EV), Flag-tagged mitoCERT, or Flag-tagged mitoCERTΔSTART. At 24 h post transfection, cells were processed for immunoblotting with antibodies against PARP1, cleaved caspase-3 (Casp3), the Flag-epitope, the HA-epitope, and β-actin. FL full-length, CL cleaved. b Quantitative analysis of PARP1 cleavage in cells treated as in a. Data are means ± s.d.; n = 4; *p < 0.05, **p < 0.01 and ***p < 0.001 by two-tailed paired t-test. c Quantitative analysis of cleaved Casp3 in cells treated as in a. Data are means ± s.e.; n = 2. Source data