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. 2019 Apr 17;10:845. doi: 10.3389/fimmu.2019.00845

Figure 1.

Figure 1

LH2171 inhibits production of inflammatory cytokines in LPS-stimulated peritoneal macrophages. The peritoneal macrophages were pre-incubated with or without LH2171 for 4 h and subsequently stimulated with 1 μg/mL LPS for 16 h. The levels of IL-6 in the supernatant was determined by ELISA (A). LPS-stimulated macrophages were additionally treated with 5 μM nigericin for 45 min to measure IL-1β level in the cell culture supernatant (B). The peritoneal macrophages were pre-incubated with different lactic acid bacteria for 4 h and stimulated with 1 μg/mL LPS for 16 h, and subsequently, IL-6 level in the culture supernatant was measured (C). LPS-stimulated macrophages were additionally treated with 5 μM nigericin for 45 min to measure IL-1β level in the cell culture supernatant (D). The concentration of LH2171 or other lactic acid bacteria was 10 μg/mL unless otherwise indicated. Data are shown as mean + SD (n = 3) and analyzed by Tukey-Kramer's (a−cP < 0.05) (A) or Dunnett's test (*P < 0.05, **P < 0.01) (B–D), which compared the LPS-treated group (C) or LPS + nigericin-treated group (B,D) with the other groups.