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. 2019 Apr 17;10:845. doi: 10.3389/fimmu.2019.00845

Figure 4.

Figure 4

LH2171 induced the expression of negative regulators of NF-κB/MAPKs signaling in peritoneal macrophages. The peritoneal macrophages were pre-incubated with or without 10 μg/mL LH2171 for 30 min, 1 and 4 h, after which Tollip, Irakm, A20, Socs1, and Socs3 mRNAs were quantified by qPCR assay (A). The peritoneal macrophages were pre-incubated with or without 10 μg/mL LH2171 for 4 h, after which A20, Socs1, Socs3, and β-tubulin were detected by western blot analysis (B). Densitometric quantification of A20/β-tubulin ratio, Socs1/β-tubulin ratio, and Socs3/β-tubulin ratio was shown (C). The quantified values were determined relative to the mean values of untreated control at 0 h (A) or at 4 h (C). Data are shown as mean + (±) SD (n = 3) and analyzed by Student's t-test (**P < 0.01), which compared the LH2171-treated group with untreated control at the same time point.